Flow Cytometry Laboratory - NUS Medical Sciences Cluster

Equipment

Equipment

Cell sorters

Beckman Coulter Moflo Astrios

MoFlo Astrios
  • System: Jet-in-air
  • Sorting proficiency: 6-way, combination of 5-50ml tubes, 96-well plate and 384-well plate
  • Enhanced forward scatter (eFSC) for improved overall particle size range with resolution up to 0.2um

 

Excitation laser Emission wavelength Common dyes/antibodies
405nm (Violet) 405/10 SSC
448/59 CFP, DAPI, Pacific Blue, BV421, V450
546/20 AmCyan, V500
576/21 Qdot 585
614/20 Qdot610
671/30 Qdot 655
488nm (Blue)

                                          

488/6 SSC
513/26 FITC, GFP, AF488
710/45 PerCP, PerCP-Cy5.5
561nm (Yellow Green) 579/16 PE, DsRed
614/20 PE-Texas Red, PE-CF594, PI, mCherry
664/22 PE-Cy5, 7AAD
692/75 PE-Cy5.5, mPlum
795/70 PE-Cy7
640nm (Red) 671/30 APC, AF647
722/44 AF700
795/70 APC-Cy7, APC-H7

Analysers

BD LSR Fortessa 

BD LSRFortessa
  • 4 lasers, 16 colors

 

Excitation laser Emission wavelength Common dyes/antibodies
405nm (Violet) 450/50 CAS B, CFP, DAPI, Pacific Blue, BV421, V450
525/50 CAS Y, AmCyan, V500
540/30 Qdot 565
585/15 Qdot 585
610/20 Qdot 605
670/30 Qdot 655
488nm (Blue)

                                          

488/10 SSC
530/30 FITC, GFP, AF488
710/50 PerCP, PerCP-Cy5.5
561nm (Yellow Green) 582/15 PE
610/20 PE-Texas Red, PE-CF594, PI, mCherry
670/30 PE-Cy5, 7AAD
710/50 PE-Cy5.5
780/60 PE-Cy7
640nm (Red) 670/14 APC, AF647
730/45 AF700
780/60 APC-Cy7, APC-H7

 

 

Beckman Coulter CyAn ADP

CyAn ADP
  • 3 lasers, 9 colors

 

Excitation laser Emission wavelength Common dyes/antibodies
405nm (Violet) 450/50 CAS B, CFP, DAPI, Pacific Blue, BV421, V450
530/40 CAS Y, AmCyan, V500, Qdot 565
488nm (Blue) 488/10 SSC
530/40 FITC, GFP, AF488
575/25 PE
613/20 PE-Texas Red, PI
680/30 PE-Cy5, PerCP, PerCP-Cy5.5, 7AAD, PE-Cy5.5
750LP PE-Cy7
635nm (Red) 665/20 APC, AF647
750LP APC-Cy7, APC-H7

 

 

Beckman Coulter CytoFLEX LX B-R-V-Y-U-I

Cyto Flex LX
  • 6 lasers, 21 colors

 

Excitation laser Emission wavelength Common dyes/antibodies
355 (UV) 405/30 BUV395
525/40 BUV496, Qdot 525, Qdot 545
675/30 BUV661, Hoechst Red
405nm (Violet) 450/45 AF405, V450, CFP, Ctyophase, Violet, eFluor 450, Pacific Blue, BV421, Zombie Violet
525/40 CAS Y, AmCyan, V500, BV510,  eCFP, Krome Orange, Zombie Aqua, LIVE/DEAD Fixable Aqua
610/20 BV605, eFlour 625NC, Qdot 605
660/10 BV650, Qdot 655
763/43 BV785, BV786, Qdot 800
488nm (Blue)

                                          

525/40 FITC, GFP, AF488, BB515, CFSE, Fluo-3, MitoSpy Green FM, Oregon Green 488, Zombie Green
610/20 PI, Mitosox
690/50 AF647-R-PE, DRAQ7, PE-Cy5, PE-Cy5.5, PerCP, PerCP-Cy5.5
561nm (Yellow Green) 585/42 PE, AF546, Cy3, DsRed, dTomato, MitoSpy Orange, CMTMRos
610/20 PE-Texas Red, PE-CF594, PI, mCherry, PE-Dazzle 594, PHrodo Red, Zombie Red
675/30 PE-Cy5, mPlum
710/50 PE-Cy5.5, PE-Cy5, PE-AF680, PerCP, PerCP-Cy5.5
763/43 PE-Cy7
638nm (Red) 660/10 AF633, AF635, AF647, APC, Cy5, eFluor 660, Flash Phalloidin NIR 647, Helix NP NIR, TO-PRO-3
712/25 AF680, AF680-APC, AF680-R-PE, AF700-APC, AF700, Cy5.5, Cy5.5-APC, DRAQ5, DRAQ7
763/43 AF750-APC, APC-eFlour 780, APC-Fire 750, APC-Cy7, APC-H7, DRAQ7, Zombie NIR
808nm (Infrared) 840/20 AF750, AF790, CF790
885/40 PF840

 

Magnetic Cell Sorter 

Miltenyi SuperMACS separator

 

  • Magnetic-based cell separator
  • Positive and negative selection for magnetically labeled and unlabeled cells, respectively
  • Useful for pre-enrichment before FACS

 

 

Software

  • FlowJo V10 (available for use at workstation, please contact us for booking or details)
  • Summit 4.3 (free version available)
  • Summit 6.3.1 (free version available)
  • BD FACS Diva
  • Beckman Coulter Kaluza (dongle for loan)
  • CytExpert (free version available)
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